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Choi, Jung-Ok
- Antimicrobial Activity of Coptischinensis Extract against Dental Caries
Authors
1 Dept. of Dental Hygiene, Hallym Polytechnic University, Chuncheon-si 24210, KP
2 Dept. of Dental Hygiene, College of Health Sciences, Youngsan University, Yangsan-si 51510, KP
3 Dept. of Dental Hygiene, College of Health Sciences, Kangwon National University, Samcheok-si 25949, KP
Source
Medico-Legal Update, Vol 20, No 1 (2020), Pagination: 1907-1912Abstract
Background/Objectives: Many microorganisms reside in the oral cavity, and they are referred to as oral-resident bacteria. Oral microorganisms are an important cause of oral diseases, and as such, many studies have been conducted to control them. This study aimed to investigate the antimicrobial activity against S. intermedius and S. gordoii among the oral-resident bacteria using Coptischinensis, a natural extract.
Method/Statistical Analysis: To investigate the antimicrobial activity of Coptischinensis, the clear-zone confirmation method using filter paper and colony-forming unit (CFU) was used to check the bacterial proliferation. As the concentration of Coptischinensis increased, the size of the clear zone also increased. As a result of the CFU, bacterial proliferation was not observed in the experimental group as compared to the control group.
Findings: The results of this study proved the antimicrobial activity of Coptischinensis against the oral-resident bacteria S. intermedius and S. gordoii. The clear zone results showed that S. intermedius and S. gordoii were larger in size than the control group.CFU showed a large amount of colony in the control group but not exist colony in the experimental group.The result showed its potential as an antimicrobial agent applicable to oral-resident bacteria.
Improvements/Applications: S. intermedius and S. gordoii bacteria found in the oral cavity showed antimicrobial effect on the extracts. Coptischinensis was confirmed to be a preventative and therapeutic agent of tooth decay. In conclusion, the possibility that it can be applied to various intraoral antibacterial products in the future.
Keywords
Antibacterial Activity, Coptischinensis, Dental Caries, Dental Pathogens, Microorganism.
- Effect of Sanitizers and Disinfectants in Staphylococcus Saprophyticus
Authors
1 College of Health Sciences, Youngsan University, Department of Dental Hygiene, KP
2 Masan University, Department of Dental Hygiene, KP
Source
Medico-Legal Update, Vol 20, No 1 (2020), Pagination: 2063-2068Abstract
Background/Objectives: In the dental environment, handpiece or ultrasonic scaler used in treatment causes aerosols and various pathogenic microorganisms. Many types of pathogens cause infectious diseases. This study is to identify surface disinfectants suitable for the management of Staphylococcus saprophyticus bacterial species, one of the causes of infection in medical institutions to use them as standardized surface management resources for infection control in dental clinic.
Method/Statistical Analysis: Commonly used 7 types of disinfectants were classified to select commercialized disinfectants. Samples were taken by rubbing the surface of the unit chair with a sterile swab. In order to check the bacterial species cultured in the medium, a single colony was purely separated by streaking. To identify the bacterial species, DNA was extracted from the bacteria and PCR was performed. the mean and standard deviation of each group. One-way ANOVA and Scheffe’s post-test were carried out to identify the clear zone of paper discs and significant differences between groups.
Findings: The test for bacterial identification was carried out for the surface flora of the unit chair. As a result, S. saprophyticus was identified on the surface. According to the result of comparative analysis of the clear zone size of each disinfectant, sodium hypochlorite (NaOCl), a chlorine disinfectant was found to have the highest disinfection effect (8.52mm), followed by Distell, an ammonium compound disinfectant (2.72).
Improvements/Applications: The result of the analysis of the clear zone of the disinfectant showed excellent antibacterial activity in 0.3% NaOCl and 0.5% Distel, indicating that they are disinfectants suitable for S. saprophyticus used in the study.